Summary: Objective. This study reports on the preliminary psychometric evaluation of the Transsexual Voice Questionnaire for Male-to-Female Transsexuals (MtF)—TVQMtF, a tool designed to measure the perceptions of MtF transsexuals regarding their voice. The TVQMtF evolved from an extensive review of the existing Transgender Selfevaluation Questionnaire (TSEQ). Study Design. This study was conducted in two phases. In phase I, the TSEQ was reviewed. In phase II, classical test theory was applied to examine two psychometric properties (internal consistency and test-retest reliability) of the TVQMtF. Method. In phase I, two speech pathologists and two MtF individuals reviewed the TSEQ, and a comparison was ade with the results of a thematic analysis of 137 voice-related extracts from the transcripts of in-depth interviews with 14 MtF individuals. In phase II, 29 Australian and six Canadian MtF transsexuals completed the new tool, the TVQMtF twice with an interim period of 4–6 weeks to enable psychometric evaluation. Results. Phase I led to substantial changes to the content and structure of the original measure, the TSEQ, and the subsequent development of the TVQMtF. In phase II, internal consistency of the TVQMtF was found to be high (time 1 Cronbach’s alpha .964; time 2 alpha .974). Excellent test-retest reliability was demonstrated (intraclass correlation coefficient alpha 0.979; 95% confidence interval alpha 0.968–0.988). Conclusions. This study provides psychometric evidence of the reliability of the TVQMtF. The MtF participants in this study reported a wide range of vocal functioning and voice-related difficulties. Voice pitch was reported as the most problematic aspect of vocal functioning, and psychosocial issues were the most frequently reported voice-related impacts.